Multimodal fiber-probe spectroscopy allows detecting epileptogenic focal cortical dysplasia in children

Year: 2017

Authors: Anand S., Cicchi R., Giordano F., Conti V., Buccoliero AM., Guerrini R., Pavone FS.

Autors Affiliation: INO CNR, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, LENS, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Anna Meyer Childrens Hosp, Div Neurosurg, Dept Neurosci 1, Viale Pieraccini 24, I-50141 Florence, Italy; Univ Florence, Anna Meyer Childrens Hosp, Neurosci Dept, Pediat Neurol Neurogenet & Neurobiol Unit & Labs, Viale Pieraccini 24, I-50139 Florence, Italy; Univ Florence, Anna Meyer Childrens Hosp, Div Pathol, Viale Pieraccini 24, I-50139 Florence, Italy; Univ Florence, Dept Phys, Via Giovanni Sansone 1, I-50019 Sesto Fiorentino, Italy.

Abstract: We evaluated the diagnostic capability of a multimodal spectroscopic approach for classifying normal brain tissue and epileptogenic focal cortical dysplasia in children. We employed fluorescence spectroscopy at two excitation wavelengths (378 nm and 445 nm) and Raman spectroscopy (at 785 nm excitation) for acquiring fluorescence and Raman spectra from 10 normal brains, 16 focal cortical dysplasia specimens and 1 cortical tuber tissue sites using a custom-built multimodal optical point spectroscopic system. We used principal component analysis combined with leave-one-sample-out-cross-validation for tissue classification. The study resulted in 100% sensitivity and 90% specificity using the information obtained from fluorescence at two distinct wavelengths and Raman spectroscopy for discriminating normal brain tissue and focal cortical dysplasia. Our results demonstrate that this methodology has the potential to be applied clinically for the detection of focal cortical dysplasia and can help to improve as precise as possible surgical resection of the dysplastic tissue during surgery for epilepsy.

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 10 (6-7)      Pages from: 896  to: 904

More Information: The research leading to these results has received funding from Fondazione Pisa in the framework of the project Diagnostic technology for the postoperative monitoring of pediatric brain tumors, from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX, from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number 284464, from the Italian Ministry of Health (GR-2011-02349626), from Tuscany Region and EU FP7 BiophotonicsPlus projects LighTPatcH (Led Technology in Photo Haemostasis) and LITE (Laser Imaging of The Eye), and from Ente Cassa di Risparmio di Firenze, from the European Union Seventh Framework Programme FP7/2007-2013 under the project ’DESIRE’ (grant agreement 602531). The authors would like to thank the operating room staff from Anna Meyer Pediatric Hospital Florence, Italy for their assistance in tissue sample collection.
KeyWords: Brain; Fluorescence; Fluorescence spectroscopy; Probes; Raman spectroscopy; Spectrum analysis; Surgery; Tissue; Tissue engineering; Transplantation (surgical), Cross validation; Diagnostic capabilities; Excitation wavelength; Focal cortical dysplasias; Optical spectroscopy; Spectroscopic systems; Surgical resection; Tissue classification, Principal component analysis, child; cortical dysplasia; diagnostic imaging; human; multimodal imaging; principal component analysis; Raman spectrometry; spectrofluorometry, Child; Humans; Malformations of Cortical Development; Multimodal Imaging; Principal Component Analysis; Spectrometry, Fluorescence; Spectrum Analysis, Raman
DOI: 10.1002/jbio.201600136

Citations: 16
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-11-17
References taken from IsiWeb of Knowledge: (subscribers only)
Connecting to view paper tab on IsiWeb: Click here
Connecting to view citations from IsiWeb: Click here