A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures

Year: 2011

Authors: Palombi L., Cecchi G., Lognoli D., Raimondi V., Toci G., Agati G.

Autors Affiliation: Istituto di Fisica Applicata Nello Carrara, CNR, Via Madonna del Piano 10, Sesto Fiorentino, Firenze 50019, Italy

Abstract: A new computational procedure to resolve the contribution of Photosystem I (PSI) and Photosystem II (PSII) to the leaf chlorophyll fluorescence emission spectra at room temperature has been developed. It is based on the Principal Component Analysis (PCA) of the leaf fluorescence emission spectra measured during the OI photochemical phase of fluorescence induction kinetics. During this phase, we can assume that only two spectral components are present, one of which is constant (PSI) and the other variable in intensity (PSII). Application of the PCA method to the measured fluorescence emission spectra of Ficus benjamina L. evidences that the temporal variation in the spectra can be ascribed to a single spectral component (the first principal component extracted by PCA), which can be considered to be a good approximation of the PSII fluorescence emission spectrum. The PSI fluorescence emission spectrum was deduced by difference between measured spectra and the first principal component. A single-band spectrum for the PSI fluorescence emission, peaked at about 735 nm, and a 2-band spectrum with maxima at 685 and 740 nm for the PSII were obtained. A linear combination of only these two spectral shapes produced a good fit for any measured emission spectrum of the leaf under investigation and can be used to obtain the fluorescence emission contributions of photosystems under different conditions. With the use of our approach, the dynamics of energy distribution between the two photosystems, such as state transition, can be monitored in vivo, directly at physiological temperatures. Separation of the PSI and PSII emission components can improve the understanding of the fluorescence signal changes induced by environmental factors or stress conditions on plants.

Journal/Review: PHOTOSYNTHESIS RESEARCH (PRINT)

Volume: 108 (2-3)      Pages from: 225  to: 239

More Information: This work has been partially funded by the European Space Agency under ESTEC contract number 20678/07/NL/HE and by the Regione Toscana under POR-CREO FESR 2007-2013 CTOTUS project.
KeyWords: Chlorophyll, algorithm; Article; Ficus; Kinetics; Light; Metabolism; Photosystem I; Photosystem II; Plant leaf; Radiation exposure; Regression analysis; Spectrofluorometry; Temperature, Algorithms; Chlorophyll; Ficus; Kinetics; Least-Squares Analysis; Light; Photosystem I Protein Complex; Photosystem II Protein Complex; Plant Leaves; Spectrometry, Fluorescence; Temperature, Ficus benjamina
DOI: 10.1007/s11120-011-9678-5

Citations: 37
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