Functional cardiac imaging by random access microscopy

Year: 2014

Authors: Crocini C., Coppini R., Ferrantini C., Pavone F.S., Sacconi L.

Autors Affiliation: European Laboratory for Non-Linear Spectroscopy (LENS), Florence, Italy;
Division of Pharmacology, Department “NeuroFarBa”, University of Florence, Florence, Italy;
Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy;
Department of Physics and Astronomy, University of Florence, SestoFiorentino, Italy;
National Research Council, National Institute of Optics, Florence, Italy

Abstract: Advances in the development of voltage sensitive dyes and Ca2+ sensors in combination with innovative microscopy techniques allowed researchers to perform functional measurements with an unprecedented spatial and temporal resolution. At the moment, one of the shortcomings of available technologies is their incapability of imaging multiple fast phenomena while controlling the biological determinants involved. In the near future, ultrafast deflectors can be used to rapidly scan laser beams across the sample, performing optical measurements of action potential and Ca2+ release from multiple sites within cardiac cells and tissues. The same scanning modality could also be used to control local Ca2+ release and membrane electrical activity by activation of caged compounds and light-gated ion channels. With this approach, local Ca2+ or voltage perturbations could be induced, simulating arrhythmogenic events, and their impact on physiological cell activity could be explored. The development of this optical methodology will provide fundamental insights in cardiac disease, boosting new therapeutic strategies, and, more generally, it will represent a new approach for the investigation of the physiology of excitable cells.

Journal/Review: FRONTIERS IN PHYSIOLOGY

Volume: 5      Pages from: 403-1  to: 403-6

More Information: The research leading to this work received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreements 241577, 241526, and 284464. This work was also supported by the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX and by Telethon-Italy (GGP13162).
KeyWords: microscopy; fluorescence; calcium imaging; voltage-sensitive dye imaging; optical stimulation; channelrhodopsin
DOI: 10.3389/fphys.2014.00403

Citations: 10
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