Common-path configuration in total internal reflection digital holography microscopy

Year: 2014

Authors: Calabuig A., Matrecano M., Paturzo M., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, via Campi Flegrei 34, 80078-Pozzuoli (NA), Italy

Abstract: Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell-substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate-prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique’s validity, some experiments are performed to establish the advantage of this compact and simple configuration, in which the reference arm in the setup is avoided.

Journal/Review: OPTICS LETTERS

Volume: 39 (8)      Pages from: 2471  to: 2474

More Information: This research was funded by the MIUR (Italian Ministry for Research and University) within the PON project PANDION PON01_00375 Studio di sottosistemi funzionali innovativi per impieghi spaziali.
KeyWords: Surface-plasmon-resonance; Phase Microscopy; Imaging-system; Interferometry; Microfluidics; Fluorescence; Channels; Devices
DOI: 10.1364/OL.39.002471

Citations: 32
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