Particle tracking by full-field complex wavefront subtraction in digital holography microscopy

Year: 2014

Authors: Miccio L., Memmolo P., Merola F., Fusco S., Embrione V., Paciello A., Ventre M., Netti P.A., Ferraro P.

Autors Affiliation: CNR – National Institute of Optics –80078 Pozzuoli Italy; IIT @CRIB, Istituto Italiano di Tecnologia, 80125 (NA), Italy; DCMIPE, University of Naples Federico II, 80125 Naples

Abstract: The 3D tracking of micro-objects, based on digital holography, is proposed through the analysis of the complex wavefront of the light scattered by the micro-samples. Exploiting the advantages of the off-axis full-field holographic interferometry, the tracking of multiple objects is achieved by a direct wavefront analysis at the focal plane overcoming the limitation of the conventional back focal plane interferometry in which only one object at a time can be tracked. Furthermore, the method proposed and demonstrated here is a step forward with respect to other holographic tracking tools. The approach is tested in two experiments, the first investigates the Brownian motion of particles trapped by holographic optical tweezers, while the second relates to the cell motility in a 3D collagen matrix, thus showing its usefulness for lab-on-chip systems in typical bioassay testing.

Journal/Review: LAB ON A CHIP

Volume: 14 (6)      Pages from: 1129  to: 1134

More Information: Supported by Progetto Bandiera La Fabbrica del Futuro – Plastic lab-on-chips for the optical manipulation of single cells (PLUS) and by Progetto Smart Cities – Acquasystem: Procedure e tecnologie innovative per una gestione pianificata ed integrata delle risorse idriche, l’ottimizzazione energetica ed il controllo della qualita nel Ciclo Integrato delle Acque.
KeyWords: digital holography; 3D tracking; on-a-chip; optical tweezers; 3D tracking
DOI: 10.1039/c3lc51104a

Citations: 57
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