Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization

Year: 2011

Authors: Miccio L., Finizio A., Puglisi R., Balduzzi D., Galli A., Ferraro P.

Autors Affiliation: Istituto Nazionale di Ottica del CNR, (INO-CNR) – Via Campi Flegrei,34 80078 Pozzuoli Napoli,Italy; Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027 — Rivolta d’Adda, Cremona, Italy

Abstract: Differential image contrast (DIC), through the numerical managing and manipulation of complex wavefronts obtained by digital holography (DH), is investigated. We name the approach Dynamical Differential Holographic Image Contrast (DDHIC). DDHIC dispenses from special optics and/or complex setup configurations with moveable components, as usually occurs in classical DIC, that is not well-suited for investigating objects experiencing dynamic evolution during the measurement. In fact, the technique presented here, is useful for floating samples since it allows, from a single recording, to set a posteriori the best conditions for DIC imaging in conjunction with the numerical focusing feature of DH. By DDHIC, the movies can be easily built-up to offering dynamic representation of phase-contrast along all directions, thus improving the visualization. Furthermore, the dynamic representation is useful for making the proper choice of other key parameters of DIC such as the amount of shear and the bias, with the aim to optimize the visualized phase-contrast imaging as favorite representation for bio-scientists. Investigation is performed on various biological samples. (C) 2011 Optical Society of America

Journal/Review: BIOMEDICAL OPTICS EXPRESS

Volume: 2 (2)      Pages from: 331  to: 344

More Information: This work was supported in part by the project SESSIBOV 9106/7303/2009 of the Ministero delle Politiche Agricole e Forestali.
KeyWords: Interference; Interferometry; Reconstruction
DOI: 10.1364/BOE.2.000331

Citations: 67
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