On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Year: 2012

Authors: Memmolo P., Iannone M., Ventre M., Netti P.A., Finizio A., Paturzo M., Ferraro P.

Autors Affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di tecnologia, Largo Barsanti e Matteucci 53, 80125 Napoli, Italy;
CNR-Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
Department of Materials and Production Engineering University of Naples Federico II P.le Tecchio 80, 80125 Napoli, Italy

Abstract: Digital Holography (DH) in microscopic configuration is a powerful tool for the imaging of micro-objects contained into a three dimensional (3D) volume, by a single-shot image acquisition. Many studies report on the ability of DH to track particle, microorganism and cells in 3D. However, very few investigations are performed with objects that change severely their morphology during the observation period. Here we study DH as a tool for 3D tracking an osteosarcoma cell line for which extensive changes in cell morphology are associated to cell motion. Due to the great unpredictable morphological change, retrieving cell’s position in 3D can become a complicated issue. We investigate and discuss in this paper how the tridimensional position can be affected by the continuous change of the cells. Moreover we propose and test some strategies to afford the problems and compare it with others approaches. Finally, results on the 3D tracking and comments are reported and illustrated.

Journal/Review: OPTICS EXPRESS

Volume: 20 (27)      Pages from: 28485  to: 28493

More Information: This work is supported by the Progetto Operativo Nazionale (PON) project MONitoraggio Innovativo per le Coste e l’Ambiente Marino (MONICA) funded by Italian Ministry of Education, University and Research (MIUR).
KeyWords: Phase-contrast Microscopy; Digital Holography; 3-dimensional Tracking; Amplitude Analysis; Migration; Gradient; Objects
DOI: 10.1364/OE.20.028485

Citations: 63
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