In-vivo multiphoton imaging of collagen remodeling after microablative fractional rejuvenation

Year: 2011

Authors: Cicchi R., Kapsokalyvas D., Troiano M., Campolmi P., Morini C., Cosci A., Massi D., Lotti T., Pavone F.S.

Autors Affiliation: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence,
1 Via Nello Carrara, 50019 Sesto Fiorentino, Italy
Interuniversitary Center for Biological and Psychosomatic Dermatology (CIDEBIP) and Department of Dermatological Science, University of Florence, Florence, Italy;
Department of Critical Care Medicine and Surgery, University of Florence, Florence, Italy

Abstract: The potential of multiphoton microscopy in providing in-vivo early diagnosis of skin lesions has already been demonstrated, while its capability in therapy follow-up has not been deeply explored so far. Two-photon excited fluorescence and second-harmonic generation microscopy were used in combination to follow-up collagen remodeling after laser micro-ablative rejuvenation. Treated regions of volunteers were imaged with multiphoton microscopy before and after treatment, and we found a strong age-dependence of the treatment effectiveness. In particular, the photorejuvenating effect was negligible in young subjects (< 30 years), whereas a significant production of new collagen was observed in aged subjects (> 70 years). Quantification of the amount of newly produced collagen and its organization were performed by means of visual examination of two-photon images. The obtained results demonstrate the performance of laser fractional micro-ablative rejuvenation without the need of an invasive biopsy as well as the wide applicability range of applications for multiphoton microscopy in clinical dermatology.

Conference title: Photonics West – BIOS 2011
Place: San Francisco, US

More Information: The authors thank M. Giuntini, M. De Pas, A. Montuori (Electronic Workshop of LENS), R. Ballerini, A. Hajeb (Mechanical Workshop of LENS) for their help in building microscope mechanics and electronics. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement #228334.
KeyWords: Age dependence; Before and after; Early diagnosis; In-vivo; Multi-photon microscopy; Multiphoton imaging; Nonlinear microscopy; Second harmonic generation; Skin lesion; Two photon; Two-photon excited fluorescence; Visual examination, Collagen; Endocrinology; Photons, Dermatology
DOI: 10.1117/12.873684

Citations: 1
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