From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Year: 2013

Authors: Cicchi R., Vogler N., Kapsokalyvas D., Dietzek B., Popp J., Pavone FS.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6, 50125 Florence, Italy; Institute of Photonic Technology Jena (IPHT), Albert Einstein Straße 9, 07745 Jena, Germany; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany; Department of Physics, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Italy

Abstract: Second-harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non-linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 6 (2)      Pages from: 129  to: 142

KeyWords: Architectural levels; Clinical application; Hierarchical organizations; Image patterns; Molecular organization; Molecular scale; Molecular size; Nonlinear microscopy; Optical process; Probe structures; Scoring methods; SHG polarization; Tissue architecture; Tissue imaging, Harmonic generation; Probes; Tissue, Collagen, fibrillar collagen, animal; anisotropy; automated pattern recognition; chemical structure; chemistry; connective tissue; Fourier analysis; human; light related phenomena; metabolism; methodology; microscopy; molecular imaging; multiphoton microscopy; polarization microscopy; review; statistics; theoretical model; tissue distribution; ultrastructure, Animals; Anisotropy; Connective Tissue; Fibrillar Collagens; Fourier Analysis; Humans; Microscopy; Microscopy, Fluorescence, Multiphoton; Microscopy, Polarization; Models, Theoretical; Molecular Imaging; Molecular Structure; Optical Phenomena; Pattern Recognition, Automated; Tissue Distribution
DOI: 10.1002/jbio.201200092

Citations: 163
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