A molecular imaging analysis of Cx43 association with Cdo during skeletal myoblast differentiation

Year: 2013

Authors: Nosi D., Mercatelli R., Chellini F., Soria S., Pini A., Formigli L., Quercioli F.

Autors Affiliation: Dipartimento di Anatomia, Istologia e Medicina Legale, Universita` di Firenze, Largo Brambilla 3 – Firenze, Italy;
Istituto dei Sistemi Complessi, Consiglio Nazionale delle Ricerche, via Madonna del Piano 10 – Sesto Fiorentino, Italy;
Istituto di Fisica Applicata Nello Carrara, Consiglio Nazionale delle Ricerche, via Madonna del Piano 10 – Sesto Fiorentino, Italy;
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche, Via Nello Carrara 1 – Sesto Fiorentino, Italy

Abstract: Cell-to-cell contacts are crucial for cell differentiation. The promyogenic cell surface protein, Cdo, functions as a component of multiprotein clusters to mediate cell adhesion signaling. Connexin 43, the main connexin forming gap junctions, also plays a key role in myogenesis. At least part of its effects is independent of the intercellular channel function, but the mechanisms underlying are unknown. Here, using multiple optical approaches, we provided the first evidence that Cx43 physically interacts with Cdo to form dynamic complexes during myoblast differentiation, offering clues for considering this interaction a structural basis of the channel-independent function of Cx43.

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 6 (8)      Pages from: 612  to: 621

More Information: F. Quercioli wishes to thank Ente Cassa di Risparmio di Firenze for the granted funding.
DOI: 10.1002/jbio.201200063

Citations: 14
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