Digital Holography Microscopy to enhance Phase contrast of Spermatozoa cells
Year: 2011
Authors: Miccio L., Merola F., Finizio A., Memmolo P., Coppola G., Di Caprio G., Gioffré M., Puglisi R., Balduzzi D., Galli A., Ferraro P.
Autors Affiliation: Istituto Nazionale di Ottica del CNR, (INO-CNR) Naples Section, Via Campi Flegrei,34 80078 Pozzuoli – Napoli (Italy;
CNR—Istituto per la Microelettronica e Microsistemi, Via Pietro Castellino 111, 80131 — Napoli, Italy
Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027—Rivolta d’Adda, Cremona, Italy
Abstract: To improve visualization and detection of cow spermatozoa numerical analysis is implemented starting from Digital Holographic (DH) recording. Quantitative Phase Map (QPM) and qualitative Differential Interference Contrast (DIC) imaging are performed. A further approach is described to get QPM for specimen flowing in micro-fluidic channels.
Journal/Review: JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
KeyWords: Digital holography; phase contrast;