Laser-induced forward transfer in picosecond regime for cell bioprinting
Year: 2025
Authors: Duvert L., Murru C., Al-Kattan A., Alloncle A.P., Magdinier F., Testa S., Casanova A.
Autors Affiliation: Aix Marseille Univ, Ctr Natl Rech Sci CNRS, Laser Plasmas & Procedes Photon LP3, Campus Luminy, Marseille, France; Aix Marseille Univ, Inst Natl Sante & Rech Med INSERM, Marseille Med Fenet MMG, Marseille, France.
Abstract: Based on interdisciplinary approaches, bioprinting methods aim to create and design highly organized 2D and 3D cultures. In this context, it has been more than a decade since laser-induced forward transfer (LIFT) was studied on a lab scale for its ability to transfer biomaterials, specifically bioink loaded with living cells, onto a substrate. Extreme physical and mechanical phenomena contribute to the jetting dynamic of the targeted bioink, raising a spontaneous biological question: does this process negatively affect the survival rate of transferred cells’ This study demonstrates that laser pulse durations in the range of picoseconds to nanoseconds do not directly affect cell viability, indicating that LIFT is a valuable bioprinting method for transferring living cells. Moreover, we highlight the necessity of using hydrogel coatings on the surface of the receiver substrate to guarantee optimal post-printing viability of the cells. We demonstrate that the nature of the hydrogel also contributes to the resolution of the printed pattern. Among the tested materials, Matrigel demonstrated all the qualities required to ensure successful printing and should therefore be considered for future work. Overall, the results show the suitability of our LIFT setup for printing living cells in the picosecond regime with a high survival rate, paving the way for a wide range of biological applications.
Journal/Review: INTERNATIONAL JOURNAL OF BIOPRINTING
Volume: 11 (2) Pages from: 290 to: 301
More Information: The work was funded by the French National Research Agency (ANR Medilibs and Diagem projects) together with the French Defense Innovation Agency (ANR-DGA/AID-ICELARE Project ID: ANR-20-ASTR-0004) . The PhD thesis of Lucas Duvert was co-funded by Aix-Marseille University, the French Defense Innovation Agency and the MarMaRa funding scheme. This work received support from the French government under the France 2030 investment plan, as part of the Initiative d’Excellence d’Aix-Marseille Universite – A*MIDEX AMX-23-CPJ-05. The project leading to this publication has received funding from the Excellence Initiative of Aix-Marseille University-A*Midex, a French investissement d’avenir programme AMX-19-IET-007, th rough the MarMaRa funding scheme. This work was conducted using LaMP facilities at LP3. The authors also acknowledge the Region SUD for their financial support.r the France 2030 investment plan, as part of the Initiative d’Excellence d’Aix-Marseille Universite – A*MIDEX AMX-23-CPJ-05. The project leading to this publication has received funding from the Excellence Initiative of Aix-Marseille University-A*Midex, a French investissement d’avenir programme AMX-19-IET-007, through the MarMaRa funding scheme. This work was conducted using LaMP facilities at LP3. The authors also acknowledge the Region SUD for their financial support.KeyWords: Cell viability; Laser-assisted bioprinting; LIFT bioprinting; Living cells printing; Regenerative medicine; Skeletal muscle; Tissue engineeringDOI: 10.36922/ijb.7788
