Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish

Year: 2023

Authors: Turrini L., Roschi L., de Vito G., Pavone F.S., Vanzi F.

Autors Affiliation: European Lab Nonlinear Spect, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Neurosci Psychol Drug Res & Child Hlth, Viale Gaetano Pieraccini 6, I-50139 Florence, Italy; Univ Florence, Interdept Ctr Study Complex Dynam, Via Giovanni Sansone 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Phys & Astron, Via Giovanni Sansone 1, I-50019 Sesto Fiorentino, Italy; CNR, Natl Inst Opt, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Biol, Via Madonna Piano 6, I-50019 Sesto Fiorentino, Italy.

Abstract: Zebrafish has become an essential model organism in modern biomedical research. Owing to its distinctive features and high grade of genomic homology with humans, it is increasingly employed to model diverse neurological disorders, both through genetic and pharmacological intervention. The use of this vertebrate model has recently enhanced research efforts, both in the optical technology and in the bioengineering fields, aiming at developing novel tools for high spatiotemporal resolution imaging. Indeed, the ever-increasing use of imaging methods, often combined with fluorescent reporters or tags, enable a unique chance for translational neuroscience research at different levels, ranging from behavior (whole-organism) to functional aspects (whole-brain) and down to structural features (cellular and subcellular). In this work, we present a review of the imaging approaches employed to investigate pathophysiological mechanisms underlying functional, structural, and behavioral alterations of human neurological diseases modeled in zebrafish.

Journal/Review: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Volume: 24 (12)      Pages from: 9833-1  to: 9833-23

More Information: The results of our group presented in this review have been achieved thanks to the following funding: EU program H2020 EXCELLENT SCIENCE-Eur opean Research Council (ERC) under grant agreement n.692943 (BrainBIT) and n.966623 (DAPTOMIC); EU H2020 Framework Programme for Research and Innovation under specific grant agreement n.785907 (Human Brain Project SGA2) and n.945539 (Human Brain Project SGA3); Bank Foundation Fondazione Cassa di Risparmio di Firenze with grant Human Brain Optical Mapping; Italian Ministry for Education, University, and Research in the framework of the Advanced Lightsheet Microscopy Italian Node of Euro-Bioimaging ERIC.
KeyWords: zebrafish; functional imaging; behavior; neurological disorders; brain disease models; epilepsy; Alzheimerīs; Parkinsonīs; autism spectrum disorders; myelination
DOI: 10.3390/ijms24129833

Citations: 6
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