Removing striping artifacts in light-sheet fluorescence microscopy: a review
Year: 2022
Authors: Ricci P., Gavryusev V., Mullenbroich C., Turrini L., de Vito G., Silvestri L., Sancataldo G., Pavone F.S.
Autors Affiliation: European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Phys & Astron, I-50019 Sesto Fiorentino, Italy; Univ Glasgow, Sch Phys & Astron, Glasgow G12 8QQ, Lanark, Scotland; Univ Florence, Dept Neurosci Psychol Drug Res & Child Hlth, I-50139 Florence, Italy; CNR, Natl Inst Opt, I-50019 Sesto Fiorentino, Italy; Univ Palermo, Dept Phys & Chem, I-90128 Palermo, Italy.
Abstract: In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and exvivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented along the light sheet propagation direction. Several methods have been developed to address this issue, ranging from fully optical solutions to entirely digital post-processing approaches. In this work, we present them, outlining their advantages, performance and limitations. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Journal/Review: PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
Volume: 168 Pages from: 52 to: 65
More Information: This project has received funding from the European Union’s Horizon 2020 research and innovation Framework Programme under grant agreements No. 720270 (HBP-SGA1), 785907 (HBPSGA2), 945539 (HBP-SGA3), and 871124 (Laserlab-Europe), and from the EU program H2020 EXCELLENT SCIENCE -European Research Council (ERC) under grant agreement n. 692943 (BrainBIT). This research has also been supported by the Advanced Lightsheet Microscopy Italian Mode of Euro-Bioimaging ERIC, by Progetto ordinario di Ricerca Finalizzata del Ministero della salute RF-2013-02355240 and by Ente Cassa di Risparmio di Firenze (private foundation). GS is funded by PON AIM 1809078e1. VG and MCM are funded each by a Marie Sklodowska-Curie fellowship (MSCAeIFeEF-ST MesoBrainMicr and Optoheart, grant agreements No. 793849 and 842893, respectively).KeyWords: Light-sheet microscopy; Striping; 3D microscopy; Brain imagingDOI: 10.1016/j.pbiomolbio.2021.07.003Citations: 31data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-11-24References taken from IsiWeb of Knowledge: (subscribers only)