Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation
Year: 2016
Authors: Credi C., De Marco C., Molena E., Roca M.P., Martn J.S., Marques J., Fernandez-Busquets X., Levi M., Turri S.
Autors Affiliation: Politecn Milan, Dipartimento Chim Mat & Ingn Chim Giulio Natta, Piazza Leonardo da Vinci 32, I-20133 Milan, Italy; Inst Bioengn Catalonia IBEC, Nanobioengn Grp, Baldiri Reixac 10-12, Barcelona 08028, Spain; Biomed Res Networking Ctr Bioengn Biomat & Nanome, Maria de Luna 11, Zaragoza 50018, Spain; Univ Barcelona, Dept Elect, Marti i Franques 1, E-08028 Barcelona, Spain; Univ Barcelona, Hosp Clin, Barcelona Inst Global Hlth ISGlobal, Rossello 149-153, E-08036 Barcelona, Spain; Univ Barcelona, Nanosci & Nanotechnol Inst IN2UB, Marti i Franques 1, E-08028 Barcelona, Spain; Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Baldiri Reixac 10-12, Barcelona 08028, Spain.
Abstract: A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays.
Journal/Review: COLLOIDS AND SURFACES B-BIOINTERFACES
Volume: 146 Pages from: 250 to: 259
More Information: This rese arch was partially supported by grants BIO2014-52872-R (Ministerio de Economia y Competitividad, Spain), which included FEDER funds, and 2014-SGR-938 (Generalitat de Catalunya, Spain).KeyWords: Polymers; Micropatterning; Antifouling; Perfluoropolyether; Photobiotin; Soft lithography; Microcontact printing; Microcontact arrayer; Heparin; MalariaDOI: 10.1016/j.colsurfb.2016.06.020Citations: 6data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-10-20References taken from IsiWeb of Knowledge: (subscribers only)