A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform

Year: 2019

Authors: Giardini F., Biasci V., Scardigli M., Pavone FS., Bub G., Sacconi L.

Autors Affiliation: European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Expt & Clin Med, Div Physiol, I-50134 Florence, Italy; CNR, Natl Inst Opt, I-50125 Florence, Italy; McGill Univ, Dept Physiol, Montreal, PQ H3A 0G4, Canada.

Abstract: Optogenetics is an emerging method that uses light to manipulate electrical activity in excitable cells exploiting the interaction between light and light-sensitive depolarizing ion channels, such as channelrhodopsin-2 (ChR2). Initially used in the neuroscience, it has been adopted in cardiac research where the expression of ChR2 in cardiac preparations allows optical pacing, resynchronization and defibrillation. Recently, optogenetics has been leveraged to manipulate cardiac electrical activity in the intact heart in real-time. This new approach was applied to simulate a re-entrant circuit across the ventricle. In this technical note, we describe the development and the implementation of a new software package for real-time optogenetic intervention. The package consists of a single LabVIEW program that simultaneously captures images at very high frame rates and delivers precisely timed optogenetic stimuli based on the content of the images. The software implementation guarantees closed-loop optical manipulation at high temporal resolution by processing the raw data in workstation memory. We demonstrate that this strategy allows the simulation of a ventricular tachycardia with high stability and with a negligible loss of data with a temporal resolution of up to 1 ms.

Journal/Review: PHYSICS EDUCATION

Volume: 2 (1)      Pages from: 7-1  to: 7-9

More Information: This work was supported by the European Union Horizon 2020 research and innovation program under grant agreement no. 654148 Laserlab-Europe, by the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX, by Ente Cassa Risparmio di Firenze (private foundation), and by FAS-Salute ToRSADE project. L.S. holds an EMBO short-term fellowship.
KeyWords: optical mapping; voltage imaging; voltage sensitive dye; optical manipulation; optogenetics; channelrhodopsin-2; cardiac electrophysiology; LabVIEW; closed-loop; real-time analysis
DOI: 10.3390/mps2010007

Citations: 5
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