Large-scale all-optical dissection of motor cortex connectivity shows a segregated organization of mouse forelimb representations

Year: 2022

Authors: Resta F., Montagni E., de Vito G., Scaglione A., Mascaro ALA., Pavone FS.

Autors Affiliation: Univ Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Phys & Astron, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Neurosci Psychol Pharmacol & Child Hlth NEURO, I-50139 Florence, Italy; Neurosci Inst, Natl Res Council, I-56124 Pisa, Italy; Natl Inst Opt, Natl Res Council, I-50019 Sesto Fiorentino, Italy.

Abstract: In rodent motor cortex, the rostral forelimb area (RFA) and the caudal forelimb area (CFA) are major actors in orchestrating the control of complex forelimb movements. However, their intrinsic connectivity and recip-rocal functional organization are still unclear, limiting our understanding of how the brain coordinates and ex-ecutes voluntary movements. Here, we causally probe cortical connectivity and activation patterns triggered by transcranial optogenetic stimulation of ethologically relevant complex movements exploiting a large-scale all-optical method in awake mice. Results show specific activation features for each movement class, providing evidence for a segregated functional organization of CFA and RFA. Importantly, we identify a sec-ond discrete lateral grasping representation area, namely the lateral forelimb area (LFA), with unique connec-tivity and activation patterns. Therefore, we propose the LFA as a distinct forelimb representation in the mouse somatotopic motor map.

Journal/Review: CELL REPORTS

Volume: 41 (6)      Pages from: 111627-1  to: 111627-21

More Information: This research was supported by the European Union’s Horizon 2020 Research and Innovation Framework Program under grant agreements 945539 (HBP-SGA3) and 785907 (HBP-SGA2) , by the EU program H2020 EXCELLENT SCIENCE – European Research Council (ERC) under grant agreement 692943 (BrainBIT) , and by the Bank Foundation Fondazione Cassa di Risparmio di Fire-nze grant Human Brain Optical Mapping.This research was also supported by the Italian Ministry for Education, University, and Research in the framework of the Advance Lightsheet Microscopy Italian Mode of Euro-bioimaging ERIC. Some objects in the graphical abstract were created with BioRender.com .
KeyWords: Neurons; Movement; Microstimulation; Mechanisms; Dynamics; Grasp; Reach
DOI: 10.1016/j.celrep.2022.111627

Citations: 7
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-11-17
References taken from IsiWeb of Knowledge: (subscribers only)
Connecting to view paper tab on IsiWeb: Click here
Connecting to view citations from IsiWeb: Click here