KHz-rate volumetric voltage imaging of the whole Zebrafish heart

Year: 2022

Authors: Sacconi L., Silvestri L., Rodriguez EC., Armstrong GAB., Pavone FS., Shrier A., Bub G.

Autors Affiliation: CNR, European Lab Nonlinear Spect, Sesto Fiorentino, Italy; CNR, Natl Inst Opt, Sesto Fiorentino, Italy; Univ Freiburg, Univ Heart Ctr, Inst Expt Cardiovasc Med, Freiburg, Germany; Univ Freiburg, Med Fac, Freiburg, Germany; Univ Florence, Dept Phys & Astron, Florence, Italy; McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ, Canada; McGill Univ, Dept Physiol, Montreal, PQ, Canada.

Abstract: Fast volumetric imaging is essential for understanding the function of excitable tissues such as those found in the brain and heart. Measuring cardiac voltage transients in tissue volumes is challenging, especially at the high spatial and temporal resolutions needed to give insight to cardiac function. We introduce a new imaging modality based on simultaneous illumination of multiple planes in the tissue and parallel detection with multiple cameras, avoiding compromises inherent in any scanning approach. The system enables imaging of voltage transients in situ, allowing us, for the first time to our knowledge, to map voltage activity in the whole heart volume at KHz rates. The high spatiotemporal resolution of our method enabled the observation of novel dynamics of electrical propagation through the zebrafish atrioventricular canal.

Journal/Review: BIOPHYSICAL REPORTS

Volume: 2 (1)      Pages from: 100046-1  to: 100046-7

More Information: G.B. acknowledges support from the Canadian Heart and Stroke Foundation and the Canadian Foundation for Innovation. L.S. acknowledges EMBO for supporting his visit to Bub’s laboratory with a short-term fellowship. A.S. acknowledges support from the Canadian Institutes of Health Research. The authors are grateful to Alex Quinn, Matthew Stoyek, Leon Glass, Michael Guevara, Claire Brown, Arjun Krishnaswami, and Giuseppe Sancataldo for scientific advice and technical support throughout the project.
KeyWords: Early Afterdepolarizations; Microscopy; Conduction; Dynamics
DOI: 10.1016/j.bpr.2022.100046

Citations: 14
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