Design and Synthesis of Novel Raman Reporters for Bioorthogonal SERS Nanoprobes Engineering

Year: 2022

Authors: Dallari C., Innocenti R., Lenci E., Trabocchi A., Pavone FS., Credi C.

Autors Affiliation: Univ Florence, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Phys, I-50019 Sesto Fiorentino, Italy; Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy; CNR, Natl Inst Opt, I-50019 Sesto Fiorentino, Italy.

Abstract: Surface-enhanced Raman spectroscopy (SERS) exploiting Raman reporter-labeled nanoparticles (RR@NPs) represents a powerful tool for the improvement of optical bio-assays due to RRs’ narrow peaks, SERS high sensitivity, and potential for multiplexing. In the present work, starting from low-cost and highly available raw materials such as cysteamine and substituted benzoic acids, novel bioorthogonal RRs, characterized by strong signal (10(3) counts with FWHM < 15 cm(-1)) in the biological Raman-silent region (>2000 cm(-1)), RRs are synthesized by implementing a versatile, modular, and straightforward method with high yields and requiring three steps lasting 18 h, thus overcoming the limitations of current reported procedures. The resulting RRs’ chemical structure has SH-pendant groups exploited for covalent conjugation to high anisotropic gold-NPs. RR@NPs constructs work as SERS nanoprobes demonstrating high colloidal stability while retaining NPs’ physical and vibrational properties, with a limit of detection down to 60 pM. RR@NPs constructs expose carboxylic moieties for further self-assembling of biomolecules (such as antibodies), conferring tagging capabilities to the SERS nanoprobes even in heterogeneous samples, as demonstrated with in vitro experiments by transmembrane proteins tagging in cell cultures. Finally, thanks to their non-overlapping spectra, we envision and preliminary prove the possibility of exploiting RR@NPs constructs simultaneously, aiming at improving current SERS-based multiplexing bioassays.

Journal/Review: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Volume: 23 (10)      Pages from: 5573-1  to: 5573-14

More Information: This work was supported by the DoptoScreen project (Fondo di Beneficenza Intesa San Paolo 2019, B/2019/0289).
KeyWords: bioorthogonal molecules; organic synthesis; gold nanostars; Raman; surface-enhanced Raman spectroscopy
DOI: 10.3390/ijms23105573

Citations: 5
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