Dual excitation spectral autofluorescence lifetime and reflectance imaging for fast macroscopic characterization of tissues

Year: 2024

Authors: Herrando AI., Castillo-Martin M., Galzerano A., Fernandez L., Vieira P., Azevedo J., Parvaiz A., Cicchi R., Shcheslavskiy VI., Silva PG., Lagarto JL.

Autors Affiliation: Champalimaud Fdn, Biophoton Platform, Ave Brasilia, P-1400038 Lisbon, Portugal; Champalimaud Fdn, Digest Unit, Ave Brasilia, P-1400038 Lisbon, Portugal; Champalimaud Fdn, Pathol Serv, Ave Brasilia, P-1400038 Lisbon, Portugal; Natl Inst Opt CNR INO, Largo Enrico Fermi 6, I-50125 Florence, Italy; Becker & Hickl GmbH, Nunsdorfer Ring 7-9, D-12277 Berlin, Germany; Privolzhsky Res Med Univ, Minina & Pozharskogo Sq 10-1, Nizhnii Novgorod 603005, Russia.

Abstract: Advancements in optical imaging techniques have revolutionized the field of biomedical research, allowing for the comprehensive characterization of tissues and their underlying biological processes. Yet, there is still a lack of tools to provide quantitative and objective characterization of tissues that can aid clinical assessment in vivo to enhance diagnostic and therapeutic interventions. Here, we present a clinically viable fiber-based imaging system combining time-resolved spectrofluorimetry and reflectance spectroscopy to achieve fast multiparametric macroscopic characterization of tissues. An essential feature of the setup is its ability to perform dual wavelength excitation in combination with recording time-resolved fluorescence data in several spectral intervals. Initial validation of this bimodal system was carried out in freshly resected human colorectal cancer specimens, where we demonstrated the ability of the system to differentiate normal from malignant tissues based on their autofluorescence and reflectance properties. To further highlight the complementarity of autofluorescence and reflectance measurements and demonstrate viability in a clinically relevant scenario, we also collected in vivo data from the skin of a volunteer. Altogether, integration of these modalities in a single platform can offer multidimensional characterization of tissues, thus facilitating a deeper understanding of biological processes and potentially advancing diagnostic and therapeutic approaches in various medical applications.

Journal/Review: BIOMEDICAL OPTICS EXPRESS

Volume: 15 (6)      Pages from: 3507  to: 3522

More Information: Fondo di Beneficenza di Intesa Sanpaolo (B/2022/0196-ALIAS) ; H2020 Excellent Science (857894-CAST) ; Russian Science Foundation (22-29-01198) .
KeyWords: Time-resolved Autofluorescence; Fluorescence Lifetime; Auto-fluorescence; Spectroscopy; Diagnosis; Microscopy
DOI: 10.1364/BOE.505220

Citations: 3
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-11-17
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