Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts
Year: 2020
Authors: Magni G., Banchelli M., Cherchi F., Coppi E., Fraccalvieri M., Rossi M., Tatini F., Pugliese AM., Degl’Innocenti DR., Alfieri D., Matteini P., Pini R., Pavone FS., Rossi F.
Autors Affiliation: CNR, Ist Fis Applicata Nello Carrara, IFAC, I-50019 Florence, Italy; Univ Florence, Sect Pharmacol & Toxicol, Dept Neurosci Psychol Drug Res & Child Hlth, I-50139 Florence, Italy; ASO Citta Salute & Sci Torino, SCDU Chirurg Plast, I-10133 Turin, Italy; EmoLED Srl, I-50019 Florence, Italy; Univ Florence, Dept Phys, I-50019 Florence, Italy; European Lab NonLinear Spect LENS, I-50019 Florence, Italy; CNR, Ist Nazl Ott, INO, I-50125 Florence, Italy.
Abstract: Keloids are an exuberant response to wound healing, characterized by an exaggerated synthesis of collagen, probably due to the increase of fibroblasts activity and to the reduction of their apoptosis rate: currently no standard treatments or pharmacological therapies are able to prevent keloid recurrence. To reach this goal, in recent years some physical treatments have been proposed, and among them the PhotoBioModulation therapy (PBM). This work analyses the effects of a blue LED light irradiation (410-430 nm, 0.69 W/cm(2) power density) on human fibroblasts, isolated from both keloids and perilesional tissues. Different light doses (3.43-6.87-13.7-20.6-30.9 and 41.2 J/cm(2)) were tested. Biochemical assays and specific staining were used to assess cell metabolism, proliferation and viability. Micro-Raman spectroscopy was used to explore direct effects of the blue LED light on the Cytochrome C (Cyt C) oxidase. We also investigated the effects of the irradiation on ionic membrane currents by patch-clamp recordings. Our results showed that the blue LED light can modulate cell metabolism and proliferation, with a dose-dependent behavior and that these effects persist at least till 48 h after treatment. Furthermore, we demonstrated that the highest fluence value can reduce cell viability 24 h after irradiation in keloid-derived fibroblasts, while the same effect is observed 48 h after treatment in perilesional fibroblasts. Electrophysiological recordings showed that the medium dose (20.6 J/cm(2)) of blue LED light induces an enhancement of voltage-dependent outward currents elicited by a depolarizing ramp protocol. Overall, these data demonstrate the potentials that PBM shows as an innovative and minimally-invasive approach in the management of hypertrophic scars and keloids, in association with current treatments.
Journal/Review: BIOMEDICINES
Volume: 8 (12) Pages from: 573-1 to: 573-19
More Information: This research was funded by EmoLED s.r.l. The work was partially supported by the Italian Ministry of Foreign Affairs and International Cooperation of Italy through the DESWEAT project (No.PGR01065) to M.B. and P.M.KeyWords: blue light; LED; photobiomodulation; skin fibrosis; keloid; fibroblast; Cytochrome C; Raman spectroscopy; patch-clampDOI: 10.3390/biomedicines8120573Citations: 19data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-10-20References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here