Mineralization of 3D osteogenic model based on gelatin-dextran hybrid hydrogel scaffold bioengineered with mesenchymal stromal cells: A multiparametric evaluation

Year: 2021

Authors: Re F.; Sartore L.; Borsani E.; Ferroni M.; Baratto C.; Mahajneh A.; Smith A.; Dey K.; Almici C.; Guizzi P.; Bernardi S.; Faglia G.; Magni F.; Russo D.

Autors Affiliation: Univ Brescia, Dept Clin & Expt Sci, Bone Marrow Transplant Unit, ASST Spedali Civili, Piazzale Spedali Civili 1, I-25123 Brescia, Italy; ASST Spedali Civili, Ctr Ric Ematooncol AIL CREA, Piazzale Spedali Civili 1, I-25123 Brescia, Italy; Univ Brescia, Dept Mech & Ind Engn, Via Branze 38, I-25123 Brescia, Italy; Univ Brescia, Dept Clin & Expt Sci, Div Anat & Physiopathol, Viale Europa 11, I-25123 Brescia, Italy; Univ Brescia, Dept Civil Environm Architectural Engn & Math DIC, Via Valotti 9, I-25123 Brescia, Italy; CNR, IMM Bologna, Via Gobetti 101, I-40129 Bologna, Italy; CNR, PRISM Lab, INO, I-25123 Brescia, Italy; Univ Milano Bicocca, Dept Med & Surg, Clin Prote & Metabol Unit, Via Raoul Follereau 3, I-20854 Vedano Al Lambro, Italy; Univ Chittagong, Fac Sci, Dept Appl Chem & Chem Engn, Chittagong 4331, Bangladesh; ASST Spedali Civili, Lab Stem Cell Manipulat & Cryopreservat, Dept Transfus Med, Piazzale Spedali Civili 1, I-25123 Brescia, Italy; ASST Spedali Civili, Orthoped & Traumatol Unit, Via Papa Giovanni XXIII 4, I-25123 Brescia, Italy; Univ Brescia, Dept Informat Engn DII, Via Branze 38, I-25123 Brescia, Italy.

Abstract: Gelatin-dextran hydrogel scaffolds (G-PEG-Dx) were evaluated for their ability to activate the bone marrow human mesenchymal stromal cells (BM-hMSCs) towards mineralization. G-PEG-Dx1 and G-PEG-Dx2, with identical composition but different architecture, were seeded with BM-hMSCs in presence of fetal bovine serum or human platelet lysate (hPL) with or without osteogenic medium. G-PEG-Dx1, characterized by a lower degree of crosslinking and larger pores, was able to induce a better cell colonization than G-PEG-Dx2. At day 28, G-PEG-Dx2, with hPL and osteogenic factors, was more efficient than G-PEG-Dx1 in inducing mineralization. Scanning electron microscopy (SEM) and Raman spectroscopy showed that extracellular matrix produced by BM-hMSCs and calcium-positive mineralization were present along the backbone of the G-PEG-Dx2, even though it was colonized to a lesser degree by hMSCs than G-PEG-Dx1. These findings were con-firmed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), detecting distinct lipidomic signatures that were associated with the different degree of scaffold mineralization. Our data show that the architecture and morphology of G-PEG-Dx2 is determinant and better than that of G-PEG-Dx1 in promoting a faster mineralization, suggesting a more favora-ble and active role for improving bone repair.

Journal/Review: MATERIALS

Volume: 14 (14)      Pages from: 3852-1  to: 3852-23

KeyWords: bone regeneration hydrogel scaffold; mesenchymal stromal cells; human platelet lysate; Raman spectroscopy; MALDI-MS
DOI: 10.3390/ma14143852

Citations: 12
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