Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Year: 2021

Authors: Rossi F., Giada Magni G., Tatini F., Banchelli M., Cherchi F., Rossi M., Coppi E., Pugliese A.M., Rossi degl’Innocenti D., Alfieri D., Pavone F.S., Pini R., Matteini P.

Autors Affiliation: Consiglio Nazl Ric CNR IFAC, Ist Fis Applicata Nello Carrara, I-50019 Florence, Italy; Univ Florence, Dept Neurosci Psychol Drug Res & Child Hlth, Sect Pharmacol & Toxicol, I-50139 Florence, Italy; EmoLED Srl, I-50019 Florence, Italy; Univ Florence, Dept Phys, I-50019 Florence, Italy; European Lab Nonlinear Spect LENS, I-50019 Florence, Italy; Consiglio Nazl Ric CNR INO, Ist Nazl Ott, I-50125 Florence, Italy.

Abstract: In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here, we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts from healthy skin and keratinocytes. Different light doses (3.43, 6.87, 13.7, 20.6, 30.9 and 41.2 J/cm2) were used to treat the cells, evidencing inhibitory and stimulatory effects following a biphasic dose behavior. Electrophysiology was used to investigate the effects on membrane currents: healthy fibroblasts and keratinocytes showed no significant differences between treated and not treated cells. Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation: a significant decrease in peak intensity of healthy fibroblast was evidenced, while it is less pronounced in keratinocytes. In conclusion, we observed that the blue LED light can be used to modulate metabolism and proliferation of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

Journal/Review: BIOMEDICINES

Volume: 9 (1)      Pages from: 41-1  to: 41-14

More Information: This research was funded by EmoLED s.r.l. The work was partially supported by the Italian Ministry of Foreign Affairs and International Cooperation of Italy through the DESWEAT project (No.PGR01065) to M.B. and P.M.
KeyWords: photobiomodulation, blue light, LED, wound healing
DOI: 10.3390/biomedicines9010041

Citations: 29
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