Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

Year: 2020

Authors: Lagarto JL., Shcheslavskiy V., Pavone FS., Cicchi R.

Autors Affiliation: Natl Res Council INO CNR, Natl Inst Opt, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; European Lab Nonlinear Spect LENS, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Becker & Hickl GmbH, Nunsdorfer Ring 7-9, D-12277 Berlin, Germany; Ptivolzhskiy Med Res Univ, Nizhnii Novgorod 603005, Russia; Univ Florence, Dept Phys, Via G Sansone 1, I-50019 Sesto Fiorentino, Italy.

Abstract: We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.

Journal/Review: OPTICS LETTERS

Volume: 45 (8)      Pages from: 2247  to: 2250

More Information: Regione Toscana (PAR FAS 2007-2013-Bando FAS Salute 2014, POR FSE 2014-2020 Giovanisi); H2020 LEIT Information and Communication Technologies (654148 Laserlab-Europe, 732111 PICCOLO).
KeyWords: Autofluorescence; Spectroscopy; Diagnosis; States
DOI: 10.1364/OL.389300

Citations: 8
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