Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes

Year: 2019

Authors: Sanzari I., Dinelli F., Humphrey E.J., Terracciano C. M., Prodromakis T.

Autors Affiliation: Univ Southampton, Fac Engn & Phys Sci, Sch Engn, Southampton SO17 1BJ, Hants, England; CNR, INO UOS A Gozzini, Area Ric Pisa S Cataldo, Via Moruzzi 1, I-56124 Pisa, Italy; Imperial Coll London, Natl Heart & Lung Inst, London, England; Univ Southampton, Zepler Inst Photon & Nanoelect, Elect Mat & Devices Res Grp, Southampton SO17 1BJ, Hants, England.

Abstract: In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaffolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaffolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and non-hybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaffolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro- and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the effects of these scaffolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.

Journal/Review: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

Volume: 103      Pages from: 109783-1  to: 109783-11

KeyWords: PDMS, Parylene C, Microgrooves, Elasticity, NRVM
DOI: 10.1016/j.msec.2019.109783

Citations: 2
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