Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction

Year: 2006

Authors: Ferraro P., Alfieri D., De Nicola S., De Petrocellis L., Finizio A., Pierattini G.

Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy; CNR – Istituto di Cibernetica \”E. Caianiello\”, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy

Abstract: Combining the concept of lateral shear interferometry (LST) within a digital holography microscope, we demonstrate that it is possible to obtain quantitative optical phase measurement in microscopy by a new single-image-processing procedure. Numerical lateral shear of the reconstructed wavefront in the image plane makes it possible to retrieve the derivative of the wavefront and remove the defocus aberration term introduced by the microscope objective. The method is tested to investigate a silicon structure and a mouse cell line. (c) 2006 Optical Society of America

Journal/Review: OPTICS LETTERS

Volume: 31(10)      Pages from: 1405  to: 1407

More Information: Il metodo è stato brevettato:“Metodo di olografia digitale a contrasto di fase quantitativo per la ricostruzione numerica di immagini, e relativo apparato” Inventori: Pietro Ferraro, Domenico Alfieri, Sergio De Nicola, Andrea Finizio, Giovanni PierattiniAssegnatario: Consiglio Nazionale delle Ricerche. Riferimento CNR 1748. Domanda n. RM2006A000226, del 21.04.2006
KeyWords: holographic
DOI: 10.1364/OL.31.001405

Citations: 144
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