Detection and visualization improvement of spermatozoa cells by digital holography
Anno: 2011
Autori: Miccio L., Finizio A., Memmolo P., Paturzo M., Merola F., Coppola G., Di Caprio G., Gioffrè M., Puglisi R., Balduzzi D., Galli A., Ferraro P.
Affiliazione autori: CNR – Istituto Nazionale di Ottica, Naples Section, Via Campi Flegrei,34 80078 Pozzuoli – Napoli (Italy);
CNR — Istituto per la Microelettronica e Microsistemi, Via Pietro Castellino 111, 80131—Napoli, Italy;
Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027—Rivolta d’Adda, Cremona, Italy
Abstract: Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it’s able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.
Giornale/Rivista: PROCEEDINGS OF SPIE
Volume: 8089 Da Pagina: 80890C A: 80890C
Parole chiavi: Digital holography; Interference Microscopy; Image enhancementDOI: 10.1117/12.889972Citazioni: 3dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-04-28Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui