Risultati scientifici

Fast multi-directional DSLM for confocal detection without striping artifacts

Anno: 2020

Autori: Ricci P., Santacaldo G., Gavryusev V., Franceschini A., Mullenbroich MC., Silvestri L., Pavone FS.

Affiliazione autori: European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy;‎ Univ Florence, Dept Phys & Astron, I-50019 Sesto Fiorentino, Italy;‎ Univ Palermo, Dept Phys & Chem, I-90128 Palermo, Italy; Natl Inst Opt, I-50019 Sesto Fiorentino, Italy; Univ Glasgow, Sch Phys & Astron, Glasgow G12 8QQ, Lanark, Scotland

Abstract: In recent years light-sheet fluorescence microscopy (LSFM) has become a cornerstone technology for neuroscience, improving the quality and capabilities of 3D imaging. By selectively illuminating a single plane, it provides intrinsic optical sectioning and fast image recording, while minimizing out of focus fluorescence background, sample photo-damage and photo-bleaching. However, images acquired with LSFM are often affected by light absorption or scattering effects, leading to un-even illumination and striping artifacts. In this work we present an optical solution to this problem, via fast multi-directional illumination of the sample, based on an acousto-optical deflector (AOD). We demonstrate that this pivoting system is compatible with confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) by using a pivoted elliptical-Gaussian beam. We tested its performance by acquiring signals emitted by specific fluorophores in several mouse brain areas, comparing the pivoting beam illumination and a traditional static one, measuring the point spread function response and quantifying the striping reduction. We observed real-time shadow suppression, while preserving the advantages of confocal detection for image contrast. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Giornale/Rivista: BIOMEDICAL OPTICS EXPRESS

Volume: 11 (6)      Da Pagina: 3111  A: 3124

Parole chiavi: LIGHT-SHEET MICROSCOPY; FLUORESCENCE MICROSCOPY; BRAIN ACTIVITY; BESSEL BEAMS; RECONSTRUCTION; SCALE
DOI: 10.1364/BOE.390916

Citazioni: 4
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2021-09-19
Riferimenti tratti da Isi Web of Knowledge: (solo abbonati)
Link per visualizzare la scheda su IsiWeb: Clicca qui
Link per visualizzare la citazioni su IsiWeb: Clicca qui

gdpr-image
This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more