Hyperspectral (fluorescence lifetime) imaging based on a UV-VIS enhanced supercontinuum source using high-order mode propagation
Authors: Taccheo S., Mercatelli R., Soria S., D\’Andrea C., Schuster K., Bassi A., Cubeddu R., Kobelke J., Mörl K., Righini G., Quercioli F.
Autors Affiliation: College of Engineering, Swansea University, Singleton Park, SA2 8PP, Swansea, United Kingdom; IFAC-CNR Istituto di Fisica Applicata Nello Carrara, Firenze, Italy; Dipartimento di Fisica, Politecnico di Milano, Piazza. L. da Vinci 32, 20133 Milano, Italy; IPHT – Institute of Photonic Technology, Albert-Einstein-Strasse 9, 07745 Jena, Germany; Istituto Nazionale Ottica, INO-CNR, Firenze, Italy
Abstract: We report on a new source able to provide probe pulses in the UV visible range and on the demonstration of its application to hyperspectral (fluorescence lifetime) imaging measurements. The source is able to generate UV (down to 300 nm) and blue light exploiting high-order mode propagation in a microstructured fiber pumped by a Ti:Sapphire laser. We believe that further optimization of pump wavelength, fiber length and fiber zero-dispersion wavelength could generate light well below 300 nm using a simple and stable set-up and become a useful tool for biomedical imaging. We demonstrated its versatility using the source for FLIM-FRET measurement a 460 nm and hyperspectral FRET-FLIM measurements.
KeyWords: Fluorescence; Industrial applications; Laser spectroscopy; Light; Medical imaging; Supercontinuum generation, Confocal laser scanning microscope; Fluorescence lifetimes; HyperSpectral; Microstructured fibers; Microstructured fibre; Supercontinuum sources; Time-resolved spectroscopy; Zero-dispersion wavelength, FibersDOI: 10.1117/12.2039001