Voltage-induced activation of mechanosensitive cation channels of leech neurons
Authors: Menconi, M.C.; Pellegrini, M.; Pellegrino, M.
Autors Affiliation: Dipartimento di Fisiologia e Biochimica “G. Moruzzi”, Universita’ di Pisa, Via S. Zeno 31, 56127, Pisa, Italy.
Scuola Normale Superiore piazza Cavalieri 7, 56126, Pisa, Italy.
Abstract: The voltage dependence of stretch-activated cation channels in leech central neurons was studied in cell-free configurations of the patch-clamp technique.
We established that stretch-activated channels excised from identified cell bodies of desheathed ganglia, as well as from neurons in culture, were slowly
and reversibly activated by depolarizing membrane potentials. Negative pressure stimuli, applied to the patch pipette during a slow periodical modulation
of membrane potential, enhanced channel activity, whereas positive pressures depressed it. Voltage-induced channel activation was observed, with soft glass
pipettes, both in inside-out and outside-out membrane patches, at negative and positive reference potentials, respectively.
The results presented in this study demonstrate that membrane depolarization induces slow activation of stretch-activated channels of leech central neurons.
This phenomenon is similar to that found in Xenopus oocytes, however, some peculiar features of the voltage dependence in leech stretch-activated channels
indicate that specific membrane-glass interactions might not necessarily be involved. Moreover, following depolarization, stretch-activated channels in
membrane patches from neurons in culture exhibited significantly shorter delay to activation (sec) than their counterparts from neurons of freshly isolated
ganglia (hundreds of sec).
Journal/Review: JOURNAL OF MEMBRANE BIOLOGY
Volume: 180 (1) Pages from: 65 to: 72
KeyWords: leech; neurons; voltage-sensitivity; stretch-activated channels; growth coneDOI: 10.1007/s002320010056Citations: 7data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-01-16References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here