Theranostic Properties of a Survivin-Directed Molecular Beacon in Human Melanoma Cells
Authors: Carpi, S.; Fogli, S.; Giannetti, A.; Adinolfi, B.; Tombelli, S.; Da Pozzo, E.; Vanni, A.; Martinotti, E.; Martini, C.; Breschi, M.C.; Pellegrino, M.; Nieri, P.; Baldini, F.
Autors Affiliation: Department of Pharmacy, University of Pisa, Pisa, Italy; Institute of Applied Physics \”Nello Carrara\”, IFAC-CNR, Sesto Fiorentino, Florence, Italy; Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy
Abstract: Survivin is an inhibitor of apoptosis overexpressed in different types of tumors and undetectable in most terminally differentiated normal tissues. In the current study, we sought to evaluate the in vitro theranostic properties of a molecular beaconoligodeoxynucleotide (MB) that targets survivin mRNA. We used laser scanning confocal microscopy to study MB delivery in living cells and real-time PCR and western blot to assess selective survivin-targeting in human malignant melanoma cells. We further assess the pro-apoptotic effect of MB by measuring internucleosomal DNA fragmentation, dissipation of mitochondrial membrane potential (MMP) and changes in nuclear morphology. Transfection of MB into A375 and 501 Mel cells generated high signal intensity from the cytoplasm, while no signal was detected in the extracellular environment and in survivin-negative cells (i. e., human melanocytes and monocytes). MB time dependently decreased survivin mRNA and protein expression in melanoma cells with the maximum effect reached at 72 h. Treatment of melanoma cells with MB induced apoptosis by significant changes in MMP, accumulation of histone-complexed DNA fragments in the cytoplasm and nuclear condensation. MB also enhanced the pro-apoptotic effect of standard chemotherapeutic drugs tested at clinically relevant concentrations. The MB tested in the current study conjugates the ability of imaging with the pharmacological silencing activity against survivin mRNA in human melanoma cells and may represent an innovative approach for cancer diagnosis and treatment.
Journal/Review: PLOS ONE
Volume: 9 (12) Pages from: 1 to: 16
More Information: This work was supported by the regional project NANOCELL – Optical Nanosensors inside cells (PAR FAS REGIONE TOSCANA Linea 1.1.a.3), by the national flagship project NANOMAX, and by Association against Melanoma Onlus (Italy). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.DOI: 10.1371/journal.pone.0114588Citations: 21data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-05-22References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here