Scientific Results

Common-path configuration in total internal reflection digital holography microscopy

Year: 2014

Authors: Calabuig A., Matrecano M., Paturzo M., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, via Campi Flegrei 34, 80078-Pozzuoli (NA), Italy

Abstract: Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell-substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate-prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique

Journal/Review: OPTICS LETTERS

Volume: 39 (8)      Pages from: 2471  to: 2474

More Information: This research was funded by the MIUR (Italian Ministry for Research and University) within the PON project PANDION PON01_00375 \”Studio di sottosistemi funzionali innovativi per impieghi spaziali\”.
DOI: 10.1364/OL.39.002471

Citations: 21
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English