Common-path configuration in total internal reflection digital holography microscopy
Authors: Calabuig A., Matrecano M., Paturzo M., Ferraro P.
Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, via Campi Flegrei 34, 80078-Pozzuoli (NA), Italy
Abstract: Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell-substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate-prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique
Journal/Review: OPTICS LETTERS
Volume: 39 (8) Pages from: 2471 to: 2474
More Information: This research was funded by the MIUR (Italian Ministry for Research and University) within the PON project PANDION PON01_00375 \”Studio di sottosistemi funzionali innovativi per impieghi spaziali\”.DOI: 10.1364/OL.39.002471Citations: 21data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-09-15References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here