Scientific Results

Common-path configuration in total internal reflection digital holography microscopy

Year: 2014

Authors: Calabuig A., Matrecano M., Paturzo M., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica Applicata, via Campi Flegrei 34, 80078-Pozzuoli (NA), Italy

Abstract: Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell-substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate-prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique

Journal/Review: OPTICS LETTERS

Volume: 39 (8)      Pages from: 2471  to: 2474

More Information: This research was funded by the MIUR (Italian Ministry for Research and University) within the PON project PANDION PON01_00375 \”Studio di sottosistemi funzionali innovativi per impieghi spaziali\”.
DOI: 10.1364/OL.39.002471

Citations: 24
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-01-23
References taken from IsiWeb of Knowledge: (subscribers only)
Connecting to view paper tab on IsiWeb: Click here
Connecting to view citations from IsiWeb: Click here

gdpr-image
This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more