A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy
Authors: Miccio L., Memmolo P., Merola F., Fusco S., Netti P. A., Ferraro P.
Autors Affiliation: CNR, Ist Nazl Ott Napoli, I-80078 Pozzuoli, Italy
Abstract: A method for 3D tracking has been developed exploiting Digital Holography features in Microscopy (DHM). In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of samples with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the amplitude refocusing in traditional tracking processes. Moreover, in biology and biomedical research fields one of the main topic is the understanding of morphology and mechanics of cells and microorganisms. Biological samples present low amplitude contrast that limits the information that can be retrieved through optical bright-field microscope measurements. The main effect on light propagating in such objects is in phase. This is known as phase-retardation or phase-shift. DHM is an innovative and alternative approach in microscopy, it
Journal/Review: PROCEEDINGS OF SPIE
Volume: 8947 Pages from: 89471L to: 89471L