A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy

Year: 2014

Authors: Miccio L., Memmolo P., Merola F., Fusco S., Netti P. A., Ferraro P.

Autors Affiliation: CNR, Ist Nazl Ott Napoli, I-80078 Pozzuoli, Italy

Abstract: A method for 3D tracking has been developed exploiting Digital Holography features in Microscopy (DHM). In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of samples with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the amplitude refocusing in traditional tracking processes. Moreover, in biology and biomedical research fields one of the main topic is the understanding of morphology and mechanics of cells and microorganisms. Biological samples present low amplitude contrast that limits the information that can be retrieved through optical bright-field microscope measurements. The main effect on light propagating in such objects is in phase. This is known as phase-retardation or phase-shift. DHM is an innovative and alternative approach in microscopy, it


Volume: 8947      Pages from: 89471L  to: 89471L

KeyWords: Optical Tweezers; Digital holography; Three-dimensional microscopy; 3D tracking; Cell Mechanics
DOI: 10.1117/12.2039698

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