3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

Year: 2014

Authors: Merola F., Miccio L., Memmolo P., Di Caprio G., Coppola G., Netti P., Ferraro P.

Autors Affiliation: CNR INO, I-80078 Pozzuoli, NA, Italy

Abstract: We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).


Volume: 8947      Pages from: 89471A  to: 89471A

KeyWords: Optical tweezers; Digital Holography; Microfluidics; Biovolume;
DOI: 10.1117/12.2039423

Connecting to view paper tab on IsiWeb: Click here