Investigation on 3D morphological changes of in vitro cells through digital holographic microscopy
Authors: Memmolo P., Miccio L., Merola F., Netti P.A., Coppola G., Ferraro P.
Autors Affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Napoli, Italy;
CNR-Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
CNR—Istituto per la Microelettronica e Microsistemi,via Pietro Castellino 111, 80131 Napoli, Italy
Abstract: We report the investigation of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps (QPMs) of biological cells by digital holographic microscopy (DHM), with the aim to analyze the 3D positions and 3D morphology together. We consider as test case for our tool the in vitro bull sperm head morphometry analysis. Extraction and measurement of various morphological parameters are performed by using two methods: the anisotropic diffusion filter, that is based on the Gaussian diffusivity function which allows more accuracy of the edge position, and the simple thresholding filter. In particular we consider the calculation of area, ellipticity, perimeter, major axis, minor axis and shape factor as a morphological parameter, instead, for the estimation of 3D position, we compute the centroid, the weighted centroid and the maximum phase values. A statistical analysis on a data set composed by N = 14 holograms relative to bovine spermatozoa and its reference holograms is reported.
Journal/Review: PROCEEDINGS OF SPIE
Volume: 8792 Pages from: 87920R to: 87920R
KeyWords: Digital holography; Three dimensional microscopy; Morphological analysisDOI: 10.1117/12.2020570Citations: 2data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-01-16References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here