Enhancing depth of focus in tilted microfluidics channels by digital holography

Year: 2013

Authors: Matrecano M., Paturzo M., Finizio A., Ferraro P.

Autors Affiliation: CNR –Istituto Nazionale di Ottica Applicata, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy

Abstract: In this Letter we propose a method to enhance the limited depth of field (DOF) in optical imaging systems, through digital holography. The proposed approach is based on the introduction of a cubic phase plate into the diffraction integral, analogous to what occurs in white-light imaging systems. By this approach we show that it is possible to improve the DOF and to recover the extended focus image of a tilted object in a single reconstruction step. Moreover, we demonstrate the possibility of obtaining well-focused biological cells flowing into a tilted microfluidic channel. (C) 2013 Optical Society of America

Journal/Review: OPTICS LETTERS

Volume: 38 (6)      Pages from: 896  to: 898

More Information: This work is supported by the Research project (MIUR). PON01_01525 MONitoraggio Innovativo per le Coste e l
DOI: 10.1364/OL.38.000896

Citations: 15
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