Scientific Results

Combining digital holographic microscopy and optical tweezers: a new route in microfluidic

Year: 2012

Authors: Miccio L., Memmolo P., Merola F., Paturzo M., Finizio A., Grilli S., Ferraro P.

Autors Affiliation: Istituto Nazionale di Ottica (CNR-INO), Via Campi Flegrei 34, 80078—Pozzuoli, Naples, Italy

Abstract: An optical configuration is realized to obtain quantitative phase-contrast maps able to characterize particles floating in a microfluidic chamber by interference microscopy. The novelty is the possibility to drive the sample and measure it thorough the same light path. That is realized by an optical setup made of two light beams coming from the same laser source. One beam provides the optical forces for driving the particle along the desired path and, at same time, it works as object beam in the digital holographic microscope (DHM). The second one acts as reference beam, allowing recording of an interference fringe pattern (i.e., the digital hologram) in an out-of-focus image plane. This work finds application in the field of micromanipulation as, the devise developed allows to operate in microfluidic chambers driving samples flowing in very small volumes. Recently, the field of optical particle micro-manipulation has had rapid growth, due to Optical Tweezers development. A particle is trapped or moved along certain trajectories according to the intensity and phase distribution of the laser beam used.


Volume: 8430      Pages from: 84300W  to: 84300W

KeyWords: laser manipulation; quantitative phase contrast;
DOI: 10.1117/12.922894

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