Scientific Results

Detection and visualization improvement of spermatozoa cells by digital holography

Year: 2011

Authors: Miccio L., Finizio A., Memmolo P., Paturzo M., Merola F., Coppola G., Di Caprio G., Gioffrè M., Puglisi R., Balduzzi D., Galli A., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica, Naples Section, Via Campi Flegrei,34 80078 Pozzuoli – Napoli (Italy);
CNR — Istituto per la Microelettronica e Microsistemi, Via Pietro Castellino 111, 80131—Napoli, Italy;
Istituto Sperimentale Italiano “Lazzaro Spallanzani”, Località La Quercia, 26027—Rivolta d’Adda, Cremona, Italy

Abstract: Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it’s able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.


Volume: 8089      Pages from: 80890C  to: 80890C

KeyWords: Digital holography; Interference Microscopy; Image enhancement
DOI: 10.1117/12.889972

Citations: 3
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