Scientific Results

Twin-beams digital holography for 3D tracking and quantitative phase-contrast microscopy in microfluidics

Year: 2011

Authors: Memmolo P., Finizio A., Paturzo M., Miccio L., Ferraro P.

Autors Affiliation: CNR – Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
DIBET Università degli Studi di Napoli “Federico II”, via Claudio 21, 80125 Napoli, Italy

Abstract: We report on a compact twin-beam interferometer that can be adopted as a flexible diagnostic tool in microfluidic platforms with twofold functionality. The novel configuration allows 3D tracking of micro-particles and, at same time, can simultaneously furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. Experimental demonstration is given on for in vitro cells in a microfluidic environment. (C) 2011 Optical Society of America

Journal/Review: OPTICS EXPRESS

Volume: 19 (25)      Pages from: 25833  to: 25842

KeyWords: Digital holography; Image processing; Interference microscopy; Particles; Three-dimensional microscopy
DOI: 10.1364/OE.19.025833

Citations: 38
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