Scientific Results

Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy

Year: 2011

Authors: Memmolo P., Di Caprio G., Distante C., Paturzo M., Puglisi R., Balduzzi D., Galli A., Coppola G., Ferraro P.

Autors Affiliation: CNR — Istituto Nazionale di Ottica, via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
CNR — Istituto Nazionale di Ottica, viale della Libertà 3, 73010 Arnesano (LE), Italy;
DIBET, Università degli Studi di Napoli “Federico II”, via Claudio 21, 80125 Napoli, Italy;
CNR — Istituto per la Microelettronica e Microsistemi,via Pietro Castellino 111, 80131 Napoli, Italy;
Istituto Sperimentale Italiano “Lazzaro Spallanzani”, località La Quercia, 26027 Rivolta d’Adda (CR), Italy

Abstract: An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques. Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data. (C) 2011 Optical Society of America

Journal/Review: OPTICS EXPRESS

Volume: 19 (23)      Pages from: 23215  to: 23226

KeyWords: Cell analysis; Phase retrieval; Interference microscopy; Digital holography; Digital image processing
DOI: 10.1364/OE.19.023215

Citations: 55
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