Simultaneous Multiplane Imaging in Digital Holographic Microscopy
Authors: Paturzo M., Finizio A., Ferraro P.
Autors Affiliation: CNR – Istituto Nazionale di Ottica, 80078 Pozzuoli, NA, Italy
Abstract: Reduced depth of field is a severe limitation in microscopy since, at high magnification, only a portion of the imaged volume along the optical axis is in good focus at once. We demonstrate that, adopting a numerical quadratic deformed diffraction grating into the reconstruction process of digital holograms, it is possible to image three planes at different depths simultaneously, gaining flexibility dispensing with a physical grating. Moreover, multiplexed holograms recorded with two different wavelengths can be obtained in focus simultaneously by a single reconstruction. Description of the method and applications for macroscopic and microscopic biological samples are reported.
Journal/Review: JOURNAL OF DISPLAY TECHNOLOGY
Volume: 7 (1) Pages from: 24 to: 28
More Information: Manuscript received July 12, 2010; revised October 05, 2010; accepted October 05, 2010. Date of current version January 12, 2011. The research leading to these results has beedn supported by the European Community\’s Seventh Framework Programme FP7/2007-2013 under Grant Agreement 216105 (\”Real 3D\” Project)KeyWords: digital holography; extended depth of focus (EFI); diffraction gratings; extended depth; fieldDOI: 10.1109/JDT.2010.2087007Citations: 13data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2019-12-08References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here