Scientific Results

Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase

Year: 2011

Authors: Esposito R., Della Ventura B., De Nicola S., Altucci C., Velotta R., Mita D.G., Lepore M.

Autors Affiliation: CNISM-Dipartimento Scienze Fisiche, Università di Napoli Federico II, Napoli 80126, Italy;
Dipartimento di Medicina Sperimentale, Seconda Università di Napoli, Napoli 80138, Italy;
CNR – Istituto Nazionale di Ottica, compr. A. Olivetti, Via Campi Flegrei 34, Pozzuoli 80078, Italy;
Institute of Genetics and Biophysics, CNR, Naples 80131, Italy

Abstract: A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM.

Journal/Review: SENSORS

Volume: 11 (4)      Pages from: 3483  to: 3497

KeyWords: biosensors; fluerescence; Glucose Oxidase
DOI: 10.3390/s110403483

Citations: 24
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English