Scientific Results

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Author: Silvestri Ludovico 

1) Autofluorescence enhancement for label‑free imaging of myelinated fibers in mammalian brains in SCIENTIFIC REPORTS
By: Costantini I., Baria E., Sorelli M., Matuschke F., Giardini F., Menzel M., Mazzamuto G., Silvestri L., Cicchi R., Amunts K., Axer M., Pavone F.S. Year: 2021 (Cit.: 0 DOI: https://doi.org/10.1038/s41598-021-86092-7)

2) Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy in BIOMEDICAL OPTICS EXPRESS
By: de Vito G., Ricci P., Turrini L., Gavryusev V., Mullenbroich C., Tiso N., Vanzi F., Silvestri L., Pavone FS. Year: 2020 (Cit.: 1 DOI: 10.1364/BOE.396388)

3) Fast multi-directional DSLM for confocal detection without striping artifacts in BIOMEDICAL OPTICS EXPRESS
By: Ricci P., Santacaldo G., Gavryusev V., Franceschini A., Mullenbroich MC., Silvestri L., Pavone FS. Year: 2020 (Cit.: 4 DOI: 10.1364/BOE.390916)

4) Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes in FRONTIERS IN NEUROSCIENCE
By: Franceschini A., Costantini I., Pavone FS., Silvestri L. Year: 2020 (Cit.: 4 DOI: 10.3389/fnins.2020.569517)

5) Toward a High-Resolution Reconstruction of 3D Nerve Fiber Architectures and Crossings in the Brain Using Light Scattering Measurements and Finite-Difference Time-Domain Simulations in PHYSICAL REVIEW X
By: Menzel M., Axer M., De Raedt H., Costantini I., Silvestri L., Pavone FS., Amunts K., Amunts K., Michielsen K. Year: 2020 (Cit.: 6 DOI: 10.1103/PhysRevX.10.021002)

6) Deep learning strategies for scalable analysis of high-resolution brain imagery

By: Mazzamuto G., Orsini F., Roffilli M., Frasconi P., Pavone F. S., Silvestri S. Year: 2019 (Cit.: 0)

7) In-vivo and ex-vivo optical clearing methods for biological tissues: review in BIOMEDICAL OPTICS EXPRESS
By: Costantini I., Cicchi R., Silvestri L., Vanzi F., Pavone F.S. Year: 2019 (Cit.: 50 DOI: 10.1364/BOE.10.005251)

8) Tailored Sample Mounting for Light-Sheet Fluorescence Microscopy of Clarified Specimens by Polydimethylsiloxane Casting in FRONTIERS IN NEUROANATOMY
By: Di Giovanna AP., Credi C., Franceschini A., Muellenbroich MC., Silvestri L., Pavone FS. Year: 2019 (Cit.: 3 DOI: 10.3389/fnana.2019.00035)

9) Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector in JOURNAL OF BIOMEDICAL OPTICS
By: Gavryusev V., Sancataldo G., Ricci P., Montalbano A., Fornetto C., Turrini L., Laurino A., Pesce L., de Vito G., Tiso N., Vanzi F., Silvestri L., Pavone FS. Year: 2019 (Cit.: 8 DOI: 10.1117/1.JBO.24.10.106504)

10) Advanced fluorescence microscopy for in vivo imaging of neuronal activity in OPTICA
By: Sancataldo G., Silvestri L., Allegra Mascaro LA., Sacconi L., Pavone FS. Year: 2019 (Cit.: 16 DOI: 10.1364/OPTICA.6.000758)

11) Whole-Brain Vasculature Reconstruction at the Single Capillary Level (vol 8, 12573, 2018) in SCIENTIFIC REPORTS
By: Di Giovanna AP., Tibo A., Silvestri L., Mullenbroich MC., Costantini I., Allegra Mascaro AL., Sacconi L., Frasconi P., Pavone FS. Year: 2019 (Cit.: 0 DOI: 10.1038/s41598-019-42216-8)

12) Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy (vol 12, 315, 2018) in FRONTIERS IN CELLULAR NEUROSCIENCE
By: Muellenbroich MC., Turrini L., Silvestri L., Alterini T., Gheisari A., Tiso N., Vanzi F., Sacconi L., Pavone FS. Year: 2019 (Cit.: 0 DOI: 10.3389/fncel.2019.00025)

13) Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts in FRONTIERS IN NEUROANATOMY
By: Sancataldo G., Gavryusev V., de Vito G., Turrini L., Locatelli M., Fornetto C., Tiso N., Vanzi F., Silvestri L., Pavone FS.
Year: 2019 (Cit.: 9 DOI: 10.3389/fnana.2019.00007)

14) Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method
By: Costantini I., Mazzamuto G., Laurino A., Lazzari E., Sacconi L., Neri M., Simonetto A., Ruffilli M., Silvestri L., Pavone FS. Year: 2019 (Cit.: 0)

15) Reply to Entcheva: The impact of T-tubules on action potential propagation in cardiac tissue in PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
By: Scardigli M., Crocini C., Ferrantini C., Gabbrielli T., Silvestri L., Coppini R., Tesi C., Rog-Zielinska E. A., Kohl P., Cerbai E., Poggesi C., Pavone F.S., Sacconi L. Year: 2018 (Cit.: 0 DOI: 10.1073/pnas.1720253115)

16) Confocal multispot microscope for fast and deep imaging in semicleared tissues in JOURNAL OF BIOMEDICAL OPTICS
By: Adam M.P., Muellenbroich M.C., Di Giovanna A.P., Alfieri D., Silvestri L., Sacconi L., Pavone F.S. Year: 2018 (Cit.: 1 DOI: 10.1117/1.JBO.23.2.020503)

17) Whole-Brain Vasculature Reconstruction at the Single Capillary Level in SCIENTIFIC REPORTS
By: Di Giovanna A.P., Tibo A., Silvestri L., Muellenbroich M.C., Costantini I., Allegra Mascaro A.L., Sacconi L., Frasconi P., Pavone F.S. Year: 2018 (Cit.: 40 DOI: 10.1038/s41598-018-30533-3)

18) Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy in FRONTIERS IN CELLULAR NEUROSCIENCE
By: Muellenbroich M. C., Turrini L., Silvestri L., Alterini T., Gheisari A., Vanzi F., Sacconi L., Pavone F. S. Year: 2018 (Cit.: 19 DOI: 10.3389/fncel.2018.00315)

19) High-fidelity imaging in brain-wide structural studies using light-sheet microscopy in ENEURO
By: Müllenbroich M.C., Silvestri L., Di Giovanna A.P., Mazzamuto G., Costantini I., Sacconi L., Pavone F.S. Year: 2018 (Cit.: 5 DOI: 10.1523/ENEURO.0124-18.2018)

20) Towards a full volumetric atlas of cell-specific neuronal spatial organization in the entire mouse brain
By: Silvestri L., Di Giovanna A.P., Mazzamuto G., Leergard T., Orsini F., Costantini I., Bjaalie J., Frasconi P., Pavone F.S. Year: 2018 (Cit.: 0)

21) Software tools for efficient processing of high-resolution 3D images of macroscopic brain samples
By: Mazzamuto G., Silvestri L., Costantini I., Orsini F., Roffilli M., Frasconi P., Sacconi L., Pavone F.S. Year: 2018 (Cit.: 0)

22) 3D human brain digital istopatology
By: Costantini I., Mazzamuto G., Silvestri L., Neri M., Roffilli M., Conti V., Guerrini R., Sacconi L., Pavone F.S. Year: 2018 (Cit.: 0 DOI: 10.1364/BRAIN.2018.BF4C.1)

23) Automatic Segmentation of Neurons in 3D Samples of Human Brain Cortex
By: Mazzamuto G., Costantini I., Neri M., Roffilli M., Silvestri L., Pavone F.S. Year: 2018 (Cit.: 0)

24) Quantitative assessment of passive electrical properties of the cardiac T-tubular system by FRAP microscopy in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
By: Scardigli M., Crocini C., Ferrantini C., Gabbrielli T., Silvestri L., Coppini R., Tesi C., Rog-Zielinska E., Kohl P., Cerbai E., Poggesi C., Pavone F. S., Sacconi L. Year: 2017 (Cit.: 23 DOI: 10.1073/pnas.1702188114)

25) Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction
By: Costantini I., Menzel M., Silvestri L., Schubert N., Axer M., Amunts K., Pavone F.S. Year: 2017 (Cit.: 0)

26) Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains
By: Silvestri L., Müllenbroich M.C., Costantini I., Di Giovanna A.P., Sacconi L., Pavone F.S. Year: 2017 (Cit.: 0 DOI: 10.1364/BODA.2017.JTu4A.8)

27) Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy
By: Di Giovanna A.P., Tibo A., Silvestri L., Muellenbroich M.C., Costantini I., Sacconi L., Frasconi P., Pavone F.S. Year: 2017 (Cit.: 0)

28) High-fidelity imaging with bessel-beam light-sheet microscopy for whole-brain structural and functional studies
By: Mu¨llenbroich M.C., Silvestri L., Turrini L., Di Giovanna A.P., Alterini T., Gheisari A., Sacconi L., Vanzi F., Pavone F.S. Year: 2017 (Cit.: 0 DOI: 10.1364/BRAIN.2017.BrW4B.2)

29) High-fidelity functional and structural whole-brain imaging with Bessel-beam light-sheet microscopy
By: Mullenbroich MC., Silvestri L., Turrini L., Di Giovanna AP., Alterini T., Gheisari A., Ricci P., Sacconi L., Vanzi F., Pavone FS. Year: 2017 (Cit.: 0)

30) Multi-Modal Optical Imaging of the Cerebellum in Animals in CEREBELLUM
By: Allegra Mascaro A.L., Sacconi L., Silvestri. L., Knott G., Pavone F.S. Year: 2016 (Cit.: 0 DOI: 10.1007/s12311-015-0730-4)

31) Clearing of fixed tissue: a review from a microscopist’s perspective in JOURNAL OF BIOMEDICAL OPTICS
By: Silvestri L., Costantini I., Sacconi L., Pavone F.S. Year: 2016 (Cit.: 80 DOI: 10.1117/1.JBO.21.8.081205)

32) Sistema e metodo di misura della focalizzazione di uno strumento ottico
By: Silvestri L., Muellenbroich M.C., Sacconi L., Pavone F.S. Year: 2016 (Cit.: 0)

33) Towards a comprehensive understanding of brain machinery by correlative microscopy in JOURNAL OF BIOMEDICAL OPTICS
By: Allegra Mascaro A. M., Silvestri L., Sacconi L., Pavone F. S. Year: 2015 (Cit.: 10 DOI: 10.1117/1.JBO.20.6.061105)

34) A versatile clearing agent for multi-modal brain imaging in SCIENTIFIC REPORTS
By: Costantini I., Ghobril J.P., Di Giovanna A.P., Allegra Mascaro A. L., Silvestri L., Müllenbroich M.C., Onofri L., Conti V., Vanzi F., Sacconi L., Guerrini R., Markram H., Iannello G., Pavone F.S. Year: 2015 (Cit.: 140 DOI: 10.1038/srep09808)

35) Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains in NEUROPHOTONICS
By: Muellenbroich M.C., Silvestri L., Onofri L., Costantini I., Van’t Hoff M., Sacconi L., Iannello G., Pavone F.S. Year: 2015 (Cit.: 12 DOI: 10.1117/1.NPh.2.4.041404)

36) Quantitative neuroanatomy of all Purkinje cells with light sheet microscopy and high-throughput image analysis in FRONTIERS IN NEUROANATOMY
By: Silvestri L., Paciscopi M., Soda P., Biamonte F., Iannello G., Frasconi P., Pavone F. S. Year: 2015 (Cit.: 25 DOI: 10.3389/fnana.2015.00068)

37) Whole brain optical imaging
By: Silvestri L., Allegra Mascaro A.L., Costantini I., Sacconi L., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1117/12.2087339)

38) A multi modal clearing method for brain imaging
By: Costantini I., Mascaro A.L.A., Di Giovanna A.P., Silvestri L., Müllenbroich M.C., Sacconi L., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1364/BRAIN.2015.BrM3B.2)

39) A new versatile clearing method for brain imaging
By: Costantini I., Di Giovanna A.P., Allegra Mascaro A.L., Silvestri L., Müllenbroich M.C., Sacconi L., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1117/12.2076908)

40) Multiphoton microscopy in brain imaging
By: Allegra Mascaro A.L., Silvestri L., Costantini I., Sacconi L., Maco B., Knott G.W., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1117/12.2084183)

41) A versatile new technique to clear mouse and human brain
By: Costantini I., Di Giovanna A.P., Mascaro A.L.A., Silvestri L., Müllenbroich M.C., Sacconi L., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1117/12.2184289)

42) Brain-wide charting of neuronal activation maps with cellular resolution
By: Silvestri L., Rudinskiy N., Paciscopi M., Müllenbroich M.C., Costantini I., Sacconi L., Frasconi P., Hyman B.T., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1364/BRAIN.2015.BrM3B.6)

43) Brain imaging from the nano- to the macro-scale
By: Silvestri L., Allegra Mascaro A.L., Costantini I., Sacconi L., Pavone F.S. Year: 2015 (Cit.: 0 DOI: 10.1109/NER.2015.7146760)

44) Correlative two-photon and light sheet microscopy in METHODS
By: Silvestri L., Allegra Mascaro A. M., Costantini I., Sacconi L., Pavone F. S. Year: 2014 (Cit.: 23 DOI: 10.1016/j.ymeth.2013.06.013)

45) Correcting Spherical Aberrations in Confocal Light Sheet Microscopy: A Theoretical Study in MICROSCOPY RESEARCH AND TECHNIQUE
By: Silvestri L., Sacconi L., Pavone F.S. Year: 2014 (Cit.: 11 DOI: 10.1002/jemt.22330)

46) Large-scale automated identification of mouse brain cells in confocal light sheet microscopy images in BIOINFORMATICS
By: Frasconi P., Silvestri L., Soda P., Cortini R., Pavone F. S., Iannello G. Year: 2014 (Cit.: 33 DOI: 10.1093/bioinformatics/btu469)

47) Exploring the brain on multiple scales with correlative two-photon and light sheet microscopy
By: Silvestri L., Allegra Mascaro A.L., Costantini I., Sacconi L., Pavone SF. Year: 2014 (Cit.: 0 DOI: 10.1117/12.2037867)

48) Breakthroughs in Photonics 2012: Nonlinear Laser Imaging for Neuroscience in IEEE PHOTONICS JOURNAL
By: Allegra Mascaro A. L., Silvestri L., Sacconi L., Pavone FS. Year: 2013 (Cit.: 2 DOI: 10.1109/JPHOT.2013.2250941)

49) Advanced optical techniques to explore brain structure and function in JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
By: Silvestri L., Allegra Mascaro A.L., Lotti J., Sacconi L., Pavone FS. Year: 2013 (Cit.: 18 DOI: 10.1142/S1793545812300029)

50) The connectomics challenge in FUNCTIONAL NEUROLOGY
By: Silvestri L., Sacconi L., Pavone FS. Year: 2013 (Cit.: 13 DOI: 10.11138/FNeur/2013.28.3.167)

51) Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy in JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
By: Silvestri L., Bria A., Costantini I., Sacconi L., Peng H., Iannello G., Pavone F.S. Year: 2013 (Cit.: 14 DOI: 10.3791/50696)

52) Light sheet microscopy of cleared mouse brains: aberrations effects caused by refractive index mismatch
By: Silvestri L., Sacconi L., Pavone FS. Year: 2013 (Cit.: 0 DOI: 10.1117/12.2032488)

53) Neural plasticity explored by correlative two-photon and electron/spim microscopy
By: Mascaro A.L.Allegra, Silvestri L., Costantini I., Sacconi L., Maco B., Knott G.W., Pavone F.S. Year: 2013 (Cit.: 0 DOI: 10.1117/12.2032505)

54) Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain in OPTICS EXPRESS
By: Silvestri L., Bria A., Sacconi L., Iannello G., Pavone FS. Year: 2012 (Cit.: 161 DOI: 10.1364/OE.20.020582)

55) Event-driven power-law relaxations in weak turbulence in PHYSICAL REVIEW LETTERS
By: Silvestri L., Fronzoni L., Grigolini P., Allegrini P. Year: 2009 (Cit.: 54 DOI: 10.1103/PhysRevLett.102.014502)

56) Experimental Quenching of Harmonic Stimuli: Universality of Linear Response Theory in PHYSICAL REVIEW LETTERS
By: Allegrini P., Bologna M., Fronzoni L., Grigolini P., Silvestri L. Year: 2009 (Cit.: 31 DOI: 10.1103/PhysRevLett.103.030602)

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