Laser-assisted synthesis of Staphylococcus aureus protein-capped silicon quantum dots as bio-functional nanoprobes

Anno: 2013

Autori: Bagga K., Barchanski A., Intartaglia R., Dante S., Marotta R., Diaspro A., Sajti C.L., Brandi F.

Affiliazione autori: Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy;
Laser Zentrum Hannover E.V., Nanotechnology Department, Hollerithallee 8, D-30419 Hannover, Germany;
Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy;
Nanochemistry, Istituto Italiano di Tecnologia, via Morego, 30, I-16163 Genova, Italy

Abstract: A novel approach for nanofabricating protein-functionalized luminescent silicon nanoparticles based on infrared ultrafast laser ablation of silicon in an aqueous solution of Staphylococcus aureus protein A is reported. It is demonstrated that 8 nm protein A-capped silicon quantum dots with blue-green photoemissive properties are generated. The conjugation efficiency studies reveal a high percentage of protein A attached to the Si nanoparticle surface through physical adsorption phenomena during the in situ laser process. The biological functionality of laser-generated Staphylococcus aureus protein A-capped Si nanoparticles is investigated. Confocal and electron microscopy together with energy dispersive x-ray spectroscopy analysis show that these Si-based bio-nanostructures selectively bind IgG in the cells. Cell viability studies reveal that these protein A-capped Si nanoparticles are suitable for biological applications, demonstrating their potential as universal secondary biomarkers for in vivo applications such as long-term, real-time cell labeling, cell staining and controlled drug delivery.

Giornale/Rivista: LASER PHYSICS LETTERS

Volume: 10 (6)      Da Pagina: 065603-1  A: 065603-8

Maggiori informazioni: The authors gratefully acknowledge Dr Alberto Curcio for helping with the confocal microscopy experiments. AB and CLS thank the German Research Foundation within the Excellence Cluster REBIRTH for funding.
Parole chiavi: NANOPARTICLES; CELLS; PHOTOSENSITIZERS; CYTOTOXICITY; SURFACES
DOI: 10.1088/1612-2011/10/6/065603

Citazioni: 37
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2022-05-22
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