3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

Anno: 2014

Autori: Merola F., Miccio L., Memmolo P., Di Caprio G., Coppola G., Netti P., Ferraro P.

Affiliazione autori: CNR INO, I-80078 Pozzuoli, NA, Italy

Abstract: We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).

Giornale/Rivista: PROCEEDINGS OF SPIE

Volume: 8947      Da Pagina: 89471A  A: 89471A

Parole chiavi: Optical tweezers; Digital Holography; Microfluidics; Biovolume;
DOI: 10.1117/12.2039423

Link per visualizzare la scheda su IsiWeb: Clicca qui