3D visualization and biovolume estimation of motile cells by digital holography
Anno: 2014
Autori: Merola F., Miccio L., Memmolo P., Di Caprio G., Coppola G., Netti P.
Affiliazione autori: CNR-INO
CNR-IMM
IIT-CRIB
Abstract: For the monitoring of biological samples, physical parameters such as size, shape and refractive index are of crucial importance. However, up to now the morphological in-vitro analysis of in-vitro cells has been limited to 2D analysis by classical optical microscopy such as phase-contrast or DIC. Here we show an approach that exploits the capability of optical tweezers to trap and put in self-rotation bovine spermatozoa flowing into a microfluidic channel. At same time, digital holographic microscopy allows to image the cell in phase-contrast modality for each different angular position, during the rotation. From the collected information about the cell
Giornale/Rivista: PROCEEDINGS OF SPIE
Volume: 9129 Da Pagina: 91291W A: 91291W
Parole chiavi: Digital Holography; optical tweezers; microfluidics; biovolume; DOI: 10.1117/12.2052836Link per visualizzare la scheda su IsiWeb: Clicca qui