Gravity driven high throughput phase detecting cytometer based on quantitative interferometric microscopy

Anno: 2014

Autori: Xue L., Wang S., Yan K., Sun N., Ferraro P., Li Z., Liu F.

Affiliazione autori: College of Electronics and Information Engineering, Shanghai University of Electric Power,Shanghai 200090,China; Department of Information Physics & Engineering, Nanjing University of Science & Technology, Nanjing 210094,China; School of Electronic Information Engineering, Xi\’an Technological University, Xi\’an, Shaanxi 710032,China; Institute of Quality Inspection of Electronics and Electrical Appliances, Shanghai Institute of Quality Inspection and Technical Research, Shanghai 201114,China: Istituto Nazionale di Ottica Applicata del CNR, Via Campi Flegrei 34, 80078 Pozzuoli(Napoli),Italy; Single Molecule Nanometry Laboratory, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095,China

Abstract: Phase distribution detection of cells and tissues is concerned since it is an important auxiliary method for observing biological samples. High speed and large amount cell detection is needed for its high detecting efficiency. In this paper, we have proposed a simple large scale biological sample phase detection device called gravity driven high throughput phase detecting cytometer based on quantitative interferometric microscopy to obtain flowing red blood cells phase. The system could realize high throughput phase detecting and statistical analysis with high detecting speed and in real time. The statistical characteristics of red blood cells could be obtained which might be helpful for biological analysis and disease detection. We believe this method is a powerful tool to quantitatively measure the phase distribution of biological samples. (C) 2013 Elsevier B.V. All rights reserved.


Volume: 316      Da Pagina: 5  A: 9

Maggiori informazioni: This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) for Fei Liu, the National Natural Science Foundation of China (Grant no. 31372399) and the Natural Science Foundation of Jiangsu Province (Grant no. BK20130699). The authors thank Yanke Shan in Nanjing Agricultural University for his kindly help in the experiments and Dr. Haijiao Jiang in Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences for his kind help and discussions.
DOI: 10.1016/j.optcom.2013.11.040

Citazioni: 16
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2022-06-26
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